The larger the file, the larger the audience, the worse your bandwidth problem gets. You probably don't have enough bandwidth to serve a large file to a large audience, and even if you did, your bandwidth bill would go through the roof. For every download, the server consumes bandwidth equal to the size of the file. It's a gratifyingly simple approach, but it doesn't scale.

Each client then downloads the file directly from the server.

Further development of alignment software would be desirable to facilitate the application of NGS in mtDNA forensic genetics.The traditional method of distributing large files is to put them on a central server. In addition, the sequence alignment algorithm employed to align NGS reads played a significant role in the analysis of the data and the resulting mtDNA haplotypes. About two-thirds of the differences were observed in or around homopolymeric sequence stretches. A total of 64 mtGenomes (>1 million bases) were established that yielded high concordance with the corresponding STS haplotypes (<0.02% differences). We have evaluated the performance of mtGenome sequencing using the Personal Genome Machine (PGM) and compared the resulting haplotypes directly with conventional Sanger-type sequencing. Customized chemistries, laboratory workflows and data analysis packages could support the community and increase the utility of mtDNA analysis in forensics. With the emergence of Next Generation Sequencing (NGS) technologies, the body of available mtDNA data can potentially be extended much more quickly and cost-efficiently. The current method for establishing (partial) mtDNA haplotypes is Sanger-type sequencing (STS), which is laborious, time-consuming, and expensive. In addition and perhaps even more relevant in the forensic context, haplogroup-specific patterns of mutations form the basis for quality control of mtDNA sequences. In forensic genetics (partial) mtGenome information can be used to assign haplotypes to their phylogenetic backgrounds, which may, in turn, have characteristic geographic distributions that would offer useful information in a forensic case. Insights into the human mitochondrial phylogeny have been primarily achieved by sequencing full mitochondrial genomes (mtGenomes).

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